Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells.

The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine ...

Co-culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells

Mesenchymal stromal cells (MSC) have been suggested to provide a suitable cellular environment for in vitro expansion of haematopoietic stem and progenitor cells (HPC) from umbilical cord blood. In this study, we have simultaneously analysed the cell division history and immunophenotypic differentiation of HPC by using cell division tracking with carboxyfluorescein diacetate N-succinimidyl este...

Improvement of Expression of α6 and β1 Integrins by the Co-culture of Adult Mouse Spermatogonial Stem Cells with SIM Mouse Embryonic Fibroblast Cells (STO) and Growth Factors

Enhanced in vitro Cartilage Formation by Co-Culture of Human Primary Chondrocytes and Mesenchymal Stromal Cells

Collagen I Collagen II Enhanced in vitro Cartilage Formation by Co-Culture of Human Primary Chondrocytes and Mesenchymal Stromal Cells Chitrangada Acharya, Adetola Adesida, Paul Zajac, Stefan Schaeren, Marcel Jakob, Jens Riesle, Andrea Barbero, Ivan Martin. Corresponding Author: [email protected] Departments of Surgery and Biomedicine, University Hospital Basel, Switzerland.CellCoTec BV, The Neth...

Isolation of bovine spermatogonial cells and co-culture with prepubertal sertoli cells in the presence of colony stimulating factor-1

BACKGROUND: Spermatogonial stem cells (SSCs) are infrequentself-renewing cells among the type A spermatogoniawithin the seminiferous tubules and are the basis of spermatogenesisin mammalian testis. An adequate number of SSCs is aprimary requirement for the study of their behavior, regulation, andfurther biomanipulation. OBJECTIVES: In this paper, we studiedthe development of the primary co-cult...